Moreover, anthocyanins are important for the beneficial human-health effects associated with anti-inflammatory, chemopreventive, and antioxidant properties ( Dharmawansa et al., 2020 Sirilun et al., 2022). Recent studies have shown that anthocyanins could confer tolerance to low temperature, drought and high salinity stress in plants ( Zheng et al., 2019 Cirillo et al., 2021 Naing and Kim, 2021). Anthocyanins also act as sunlight attenuators, antioxidants, mediators of reactive oxygen species-induced signaling cascades, chelating agents for heavy metals, and delay leaf senescence in plants ( Naing and Kim, 2021 Zheng et al., 2021). Anthocyanins, a subclass of flavonoids, perform many biological functions in higher plants, including responsibility for a variety of colors to act as insect and animal attractants ( Landi et al., 2015 Liu et al., 2018 Liang and He, 2018). The change in leaf color is strongly associated with the synthesis and accumulation of anthocyanins in leaves ( Gao et al., 2021 Li et al., 2021). Leaf color is an important ornamental trait of cultivated colored-leaf plants. These results contribute to a systematic and comprehensive understanding of anthocyanin accumulation and to the molecular mechanisms of anthocyanin biosynthesis in QHP and ZSY.Ĭolored-leaf plants are naturally occurring, and the leaves of cultivated species differ in color from the natural green in the entire growing season. This gene was strongly correlated with 16 anthocyanin compounds, indicating that Podel.04G021100 might be involved in the regulation of anthocyanin biosynthesis. One R2R3-MYB subfamily member in the SG5 subgroup, Podel.04G021100, showed a similar expression pattern to some structural genes. Correlation analysis between RNA-seq data and metabolite profiles showed that the expression patterns of certain differentially expressed genes in the anthocyanin biosynthesis pathway were strongly correlated with the differential accumulation of anthocyanins. The major genes associated with anthocyanin biosynthesis, including structural genes and transcription factors, were differentially expressed in purple leaves of QHP and ZSY through RNA-sequencing (RNA-seq) data analysis, which was consistent with quantitative real-time PCR analysis results. Twelve cyanidins, seven pelargonidins, five delphinidins, and five procyanidins were identified as the major anthocyanin compounds, which were differentially accumulated in purple leaves of QHP and ZSY. A total of 39 anthocyanin compounds were detected by LC-MS/MS analysis.
0 kommentar(er)
